BP704T · Semester 7 · B.Pharm
Unit III — UPLC, Hyphenated Techniques and Supercritical/Flash Chromatography
Modern Analytical Techniques
This study page introduces UPLC, Hyphenated Techniques and Supercritical/Flash Chromatography in Modern Analytical Techniques (BP704T), B.Pharm Semester 7. Read the selected definitions and short notes below, then download the full unit PDF.
Topics in this unit
- UPLC and Nano-LC — Faster, Sharper Chromatography
- Hyphenated Techniques — GC-MS, LC-MS/MS, ICP-MS
- Supercritical Fluid Chromatography and Flash Chromatography
Definitions and short notes
Selected extracts from this unit’s notes. Download the full PDF below for the remaining topics and examples.
UPLC (Ultra Performance Liquid Chromatography)
A type of liquid chromatography that uses very small packing particles (smaller than 2 micrometres — much smaller than ordinary HPLC particles) and very high pressure to push the mobile phase through the column. Because the particles are so small, the separation is much faster and the peaks are much sharper than in normal HPLC.
Unit PDF · page 2
Nano-LC (Nano Liquid Chromatography)
A type of liquid chromatography that uses extremely low flow rates (measured in nanolitres per minute — thousands of times lower than HPLC) and very thin columns (about the width of a human hair). It is used when only a tiny amount of sample is available and very high sensitivity is needed.
Unit PDF · page 2
Download notes
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For education and exam preparation only, not medical advice or current regulatory guidance.